Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 67402..68138 Replicon plasmid unnamed1
Accession NZ_CP114333
Organism Klebsiella variicola strain 2022CK-00505

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag OGU18_RS27270 Protein ID WP_003026803.1
Coordinates 67656..68138 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag OGU18_RS27265 Protein ID WP_003026799.1
Coordinates 67402..67668 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OGU18_RS27230 (OGU18_27225) 63246..63608 - 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -
OGU18_RS27235 (OGU18_27230) 63660..64010 - 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
OGU18_RS27240 (OGU18_27235) 64368..64616 + 249 WP_004193994.1 hypothetical protein -
OGU18_RS27245 (OGU18_27240) 64613..65824 + 1212 WP_032415728.1 hypothetical protein -
OGU18_RS27250 (OGU18_27245) 65958..66449 + 492 WP_004206662.1 hypothetical protein -
OGU18_RS27255 (OGU18_27250) 66510..66713 + 204 WP_004206661.1 HHA domain-containing protein -
OGU18_RS27260 (OGU18_27255) 66727..66957 + 231 WP_004206660.1 hypothetical protein -
OGU18_RS27265 (OGU18_27260) 67402..67668 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
OGU18_RS27270 (OGU18_27265) 67656..68138 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
OGU18_RS27275 (OGU18_27270) 68339..69742 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
OGU18_RS27280 (OGU18_27275) 69771..70403 - 633 WP_001567369.1 hypothetical protein -
OGU18_RS27285 (OGU18_27280) 70629..71975 + 1347 WP_064152331.1 ISNCY family transposase -
OGU18_RS27290 (OGU18_27285) 72024..72419 + 396 WP_048268429.1 helix-turn-helix domain-containing protein -
OGU18_RS27295 (OGU18_27290) 72572..72682 - 111 Protein_75 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..227788 227788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T266588 WP_003026803.1 NZ_CP114333:67656-68138 [Klebsiella variicola]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT266588 WP_003026799.1 NZ_CP114333:67402-67668 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References