Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5489440..5490065 Replicon chromosome
Accession NZ_CP114324
Organism Klebsiella variicola strain 2022CK-00503

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag OEE48_RS26540 Protein ID WP_008807903.1
Coordinates 5489440..5489823 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OEE48_RS26545 Protein ID WP_004150355.1
Coordinates 5489823..5490065 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEE48_RS26525 (OEE48_26520) 5486806..5487708 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OEE48_RS26530 (OEE48_26525) 5487705..5488340 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
OEE48_RS26535 (OEE48_26530) 5488337..5489266 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OEE48_RS26540 (OEE48_26535) 5489440..5489823 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
OEE48_RS26545 (OEE48_26540) 5489823..5490065 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OEE48_RS26550 (OEE48_26545) 5490270..5491187 + 918 WP_064171510.1 alpha/beta hydrolase -
OEE48_RS26555 (OEE48_26550) 5491201..5492142 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
OEE48_RS26560 (OEE48_26555) 5492187..5492624 - 438 WP_008807906.1 D-aminoacyl-tRNA deacylase -
OEE48_RS26565 (OEE48_26560) 5492621..5493481 - 861 WP_008807907.1 virulence factor BrkB family protein -
OEE48_RS26570 (OEE48_26565) 5493475..5494074 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T266548 WP_008807903.1 NZ_CP114324:c5489823-5489440 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT266548 WP_004150355.1 NZ_CP114324:c5490065-5489823 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References