Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3904098..3904735 Replicon chromosome
Accession NZ_CP114038
Organism Bacillus velezensis strain B8

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OZA25_RS19370 Protein ID WP_003156187.1
Coordinates 3904098..3904448 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag OZA25_RS19375 Protein ID WP_003156188.1
Coordinates 3904454..3904735 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZA25_RS19330 (OZA25_19330) 3899138..3899740 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
OZA25_RS19335 (OZA25_19335) 3899740..3900528 - 789 WP_032873003.1 RNA polymerase sigma factor SigB -
OZA25_RS19340 (OZA25_19340) 3900494..3900976 - 483 WP_007609591.1 anti-sigma B factor RsbW -
OZA25_RS19345 (OZA25_19345) 3900973..3901302 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
OZA25_RS19350 (OZA25_19350) 3901366..3902373 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
OZA25_RS19355 (OZA25_19355) 3902385..3902786 - 402 WP_003156178.1 anti-sigma regulatory factor -
OZA25_RS19360 (OZA25_19360) 3902789..3903154 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
OZA25_RS19365 (OZA25_19365) 3903159..3903980 - 822 WP_003156182.1 STAS domain-containing protein -
OZA25_RS19370 (OZA25_19370) 3904098..3904448 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OZA25_RS19375 (OZA25_19375) 3904454..3904735 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OZA25_RS19380 (OZA25_19380) 3904855..3906024 - 1170 WP_032873001.1 alanine racemase -
OZA25_RS19385 (OZA25_19385) 3906141..3907148 - 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
OZA25_RS19390 (OZA25_19390) 3907313..3907678 - 366 WP_007609580.1 holo-ACP synthase -
OZA25_RS19395 (OZA25_19395) 3907771..3908370 + 600 WP_032872997.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T266223 WP_003156187.1 NZ_CP114038:c3904448-3904098 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT266223 WP_003156188.1 NZ_CP114038:c3904735-3904454 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References