Detailed information of TA system TA026517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 750228..750846
• Replicon: chromosome
• Accession: NC_017325
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: SM11_RS03525
• Protein ID: WP_010969011.1
• Coordinates: 750550..750846 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SM11_RS03520
• Protein ID: WP_010969010.1
• Coordinates: 750228..750536 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS03475	745307..745627	-	321	WP_003527320.1	YnfA family protein	-
SM11_RS34655	745702..745875	-	174	WP_010969006.1	hypothetical protein	-
SM11_RS34660	745889..746062	-	174	WP_003527318.1	hypothetical protein	-
SM11_RS03490	746298..746999	+	702	WP_014529127.1	glutathione S-transferase N-terminal domain-containing protein	-
SM11_RS03495	747223..747564	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
SM11_RS03500	748149..748415	+	267	WP_003527312.1	sel1 repeat family protein	-
SM11_RS03505	748437..749558	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
SM11_RS03515	749987..750238	-	252	WP_042708639.1	hypothetical protein	-
SM11_RS03520	750228..750536	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
SM11_RS03525	750550..750846	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM11_RS03530	750894..751199	-	306	WP_003527305.1	hypothetical protein	-
SM11_RS03535	751527..752564	+	1038	WP_003527304.1	porin	-
SM11_RS03540	752674..753741	-	1068	WP_017265823.1	site-specific integrase	-
SM11_RS03545	753782..754831	-	1050	WP_013844229.1	porin	-
SM11_RS03550	755045..755533	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T26517 WP_010969011.1 NC_017325:c750846-750550 [Sinorhizobium meliloti SM11]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T26517 NC_017325:c750846-750550 [Sinorhizobium meliloti SM11]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT26517 WP_010969010.1 NC_017325:c750536-750228 [Sinorhizobium meliloti SM11]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT26517 NC_017325:c750536-750228 [Sinorhizobium meliloti SM11]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61