Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3512482..3513152 Replicon chromosome
Accession NZ_CP112996
Organism Mycobacterium tuberculosis variant bovis strain 2018/0565

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag ORO19_RS16590 Protein ID WP_003899954.1
Coordinates 3512482..3512826 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag ORO19_RS16595 Protein ID WP_003899955.1
Coordinates 3512823..3513152 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORO19_RS16555 (ORO19_16555) 3507555..3508415 + 861 WP_003416628.1 alpha/beta hydrolase -
ORO19_RS16560 (ORO19_16560) 3508390..3508905 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
ORO19_RS16565 (ORO19_16565) 3508921..3509132 + 212 Protein_3270 (R)-hydratase -
ORO19_RS16570 (ORO19_16570) 3509145..3509438 + 294 WP_003416635.1 hypothetical protein -
ORO19_RS16575 (ORO19_16575) 3509726..3511015 + 1290 WP_003416640.1 ATP-binding protein -
ORO19_RS16580 (ORO19_16580) 3511362..3511796 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
ORO19_RS16585 (ORO19_16585) 3511799..3512251 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ORO19_RS16590 (ORO19_16590) 3512482..3512826 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ORO19_RS16595 (ORO19_16595) 3512823..3513152 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
ORO19_RS16600 (ORO19_16600) 3513690..3514037 + 348 WP_003899956.1 DUF2384 domain-containing protein -
ORO19_RS16605 (ORO19_16605) 3514034..3514654 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
ORO19_RS16610 (ORO19_16610) 3514814..3516079 - 1266 WP_003909837.1 hypothetical protein -
ORO19_RS16615 (ORO19_16615) 3516247..3516456 + 210 WP_003416778.1 hypothetical protein -
ORO19_RS16620 (ORO19_16620) 3516703..3517737 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T265035 WP_003899954.1 NZ_CP112996:3512482-3512826 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT265035 WP_003899955.1 NZ_CP112996:3512823-3513152 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References