Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2250252..2251171 Replicon chromosome
Accession NZ_CP112996
Organism Mycobacterium tuberculosis variant bovis strain 2018/0565

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag ORO19_RS10555 Protein ID WP_003900449.1
Coordinates 2250566..2251171 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag ORO19_RS10550 Protein ID WP_003410124.1
Coordinates 2250252..2250557 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORO19_RS10520 (ORO19_10520) 2245263..2246519 - 1257 WP_010950642.1 HNH endonuclease signature motif containing protein -
ORO19_RS10525 (ORO19_10525) 2246873..2247448 + 576 WP_031702535.1 hypothetical protein -
ORO19_RS10530 (ORO19_10530) 2247445..2248485 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
ORO19_RS10535 (ORO19_10535) 2248727..2249446 + 720 WP_010950643.1 DUF433 domain-containing protein -
ORO19_RS10540 (ORO19_10540) 2249436..2249852 + 417 WP_003410114.1 hypothetical protein -
ORO19_RS10545 (ORO19_10545) 2249868..2250167 - 300 WP_003410120.1 hypothetical protein -
ORO19_RS10550 (ORO19_10550) 2250252..2250557 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
ORO19_RS10555 (ORO19_10555) 2250566..2251171 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ORO19_RS10560 (ORO19_10560) 2251196..2251555 - 360 WP_003410131.1 hypothetical protein -
ORO19_RS10565 (ORO19_10565) 2251715..2252368 - 654 WP_014324107.1 hypothetical protein -
ORO19_RS10570 (ORO19_10570) 2252344..2252580 - 237 WP_003410133.1 hypothetical protein -
ORO19_RS10575 (ORO19_10575) 2252787..2253683 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T265018 WP_003900449.1 NZ_CP112996:c2251171-2250566 [Mycobacterium tuberculosis variant bovis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT265018 WP_003410124.1 NZ_CP112996:c2250557-2250252 [Mycobacterium tuberculosis variant bovis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References