Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2197884..2198752 Replicon chromosome
Accession NZ_CP112996
Organism Mycobacterium tuberculosis variant bovis strain 2018/0565

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag ORO19_RS10240 Protein ID WP_010886136.1
Coordinates 2197884..2198261 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag ORO19_RS10245 Protein ID WP_003409886.1
Coordinates 2198303..2198752 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORO19_RS10190 (ORO19_10190) 2193673..2194125 - 453 WP_010950636.1 lipoprotein -
ORO19_RS10195 (ORO19_10195) 2194189..2194590 + 402 WP_003409869.1 hypothetical protein -
ORO19_RS10200 (ORO19_10200) 2194583..2194765 - 183 WP_003409870.1 hypothetical protein -
ORO19_RS10205 (ORO19_10205) 2194879..2195229 - 351 WP_003409871.1 hypothetical protein -
ORO19_RS10210 (ORO19_10210) 2195240..2196142 - 903 WP_003409874.1 hypothetical protein -
ORO19_RS10215 (ORO19_10215) 2196163..2196354 - 192 WP_003409876.1 hypothetical protein -
ORO19_RS10220 (ORO19_10220) 2196355..2196651 - 297 WP_003409877.1 hypothetical protein -
ORO19_RS10225 (ORO19_10225) 2196891..2197106 + 216 WP_003409878.1 antitoxin -
ORO19_RS10230 (ORO19_10230) 2197103..2197414 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
ORO19_RS10235 (ORO19_10235) 2197388..2197909 - 522 WP_010950637.1 hypothetical protein -
ORO19_RS10240 (ORO19_10240) 2197884..2198261 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
ORO19_RS10245 (ORO19_10245) 2198303..2198752 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ORO19_RS10250 (ORO19_10250) 2198749..2199294 + 546 WP_003409891.1 SecB-like chaperone -
ORO19_RS10255 (ORO19_10255) 2199183..2199797 - 615 WP_003901296.1 hypothetical protein -
ORO19_RS10260 (ORO19_10260) 2199846..2200142 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
ORO19_RS10265 (ORO19_10265) 2200139..2200390 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
ORO19_RS10270 (ORO19_10270) 2200377..2200871 + 495 WP_003899099.1 hypothetical protein -
ORO19_RS10275 (ORO19_10275) 2201031..2201438 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
ORO19_RS10280 (ORO19_10280) 2201442..2201714 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ORO19_RS10285 (ORO19_10285) 2201747..2202967 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T265012 WP_010886136.1 NZ_CP112996:2197884-2198261 [Mycobacterium tuberculosis variant bovis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT265012 WP_003409886.1 NZ_CP112996:2198303-2198752 [Mycobacterium tuberculosis variant bovis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References