Detailed information of TA system TA025924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3069971..3070575
• Replicon: chromosome
• Accession: NC_016804
• Organism: Mycobacterium bovis BCG str. Mexico

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: BCGMEX_RS14540
• Protein ID: WP_003414492.1
• Coordinates: 3069971..3070363 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: BCGMEX_RS14545
• Protein ID: WP_003414495.1
• Coordinates: 3070360..3070575 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGMEX_RS14510	3065121..3065909	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
BCGMEX_RS14515	3066243..3066788	-	546	WP_003904931.1	DUF1802 family protein	-
BCGMEX_RS14520	3067060..3067944	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BCGMEX_RS14525	3067947..3068834	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
BCGMEX_RS14530	3069139..3069684	-	546	WP_003899500.1	DUF1802 family protein	-
BCGMEX_RS14535	3069681..3069950	-	270	WP_003414489.1	DUF2277 family protein	-
BCGMEX_RS14540	3069971..3070363	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGMEX_RS14545	3070360..3070575	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BCGMEX_RS14550	3070622..3071371	+	750	WP_003414497.1	enoyl-CoA hydratase	-
BCGMEX_RS14555	3071450..3072532	-	1083	WP_011799293.1	ABC transporter ATP-binding protein	-
BCGMEX_RS21260	3072525..3073835	-	1311	Protein_2841	ABC transporter substrate-binding protein	-
BCGMEX_RS14570	3073838..3074665	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
BCGMEX_RS14575	3074662..3075573	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T25924 WP_003414492.1 NC_016804:c3070363-3069971 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T25924 NC_016804:c3070363-3069971 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT25924 WP_003414495.1 NC_016804:c3070575-3070360 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT25924 NC_016804:c3070575-3070360 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8