Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2183244..2184112 Replicon chromosome
Accession NC_016804
Organism Mycobacterium bovis BCG str. Mexico

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag BCGMEX_RS10125 Protein ID WP_010886136.1
Coordinates 2183244..2183621 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag BCGMEX_RS10130 Protein ID WP_003409886.1
Coordinates 2183663..2184112 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCGMEX_RS10075 2179033..2179485 - 453 WP_010950636.1 lipoprotein -
BCGMEX_RS10080 2179549..2179950 + 402 WP_003409869.1 hypothetical protein -
BCGMEX_RS10085 2179943..2180125 - 183 WP_003409870.1 hypothetical protein -
BCGMEX_RS10090 2180239..2180589 - 351 WP_003409871.1 hypothetical protein -
BCGMEX_RS10095 2180600..2181502 - 903 WP_003409874.1 hypothetical protein -
BCGMEX_RS10100 2181523..2181714 - 192 WP_003409876.1 hypothetical protein -
BCGMEX_RS10105 2181715..2182011 - 297 WP_003409877.1 hypothetical protein -
BCGMEX_RS10110 2182251..2182466 + 216 WP_003409878.1 antitoxin -
BCGMEX_RS10115 2182463..2182774 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
BCGMEX_RS22105 2182748..2183269 - 522 WP_010950637.1 hypothetical protein -
BCGMEX_RS10125 2183244..2183621 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
BCGMEX_RS10130 2183663..2184112 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
BCGMEX_RS10135 2184109..2184654 + 546 WP_003409891.1 SecB-like chaperone -
BCGMEX_RS10140 2184543..2185157 - 615 WP_003901296.1 hypothetical protein -
BCGMEX_RS10145 2185206..2185502 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
BCGMEX_RS10150 2185499..2185750 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
BCGMEX_RS10155 2185737..2186231 + 495 WP_003899099.1 hypothetical protein -
BCGMEX_RS10160 2186391..2186798 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
BCGMEX_RS10165 2186802..2187074 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BCGMEX_RS10170 2187107..2188327 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T25905 WP_010886136.1 NC_016804:2183244-2183621 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T25905 NC_016804:2183244-2183621 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT25905 WP_003409886.1 NC_016804:2183663-2184112 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT25905 NC_016804:2183663-2184112 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References