Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 993538..994192 Replicon chromosome
Accession NZ_CP104681
Organism Escherichia coli strain 2022CK-00450

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag N5922_RS04930 Protein ID WP_000244777.1
Coordinates 993785..994192 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N5922_RS04925 Protein ID WP_000354046.1
Coordinates 993538..993804 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5922_RS04900 (988707) 988707..989450 + 744 WP_000951964.1 SDR family oxidoreductase -
N5922_RS04905 (989507) 989507..990940 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
N5922_RS04910 (990985) 990985..991296 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
N5922_RS04915 (991460) 991460..992119 + 660 WP_000250274.1 hemolysin III family protein -
N5922_RS04920 (992315) 992315..993295 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
N5922_RS04925 (993538) 993538..993804 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N5922_RS04930 (993785) 993785..994192 + 408 WP_000244777.1 protein YgfX Toxin
N5922_RS04935 (994232) 994232..994753 - 522 WP_001055874.1 flavodoxin FldB -
N5922_RS04940 (994865) 994865..995761 + 897 WP_000806628.1 site-specific tyrosine recombinase XerD -
N5922_RS04945 (995786) 995786..996496 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N5922_RS04950 (996502) 996502..998235 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T258983 WP_000244777.1 NZ_CP104681:993785-994192 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT258983 WP_000354046.1 NZ_CP104681:993538-993804 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References