Detailed information of TA system TA025891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1015435..1016054
• Replicon: chromosome
• Accession: NC_016804
• Organism: Mycobacterium bovis BCG str. Mexico

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: BCGMEX_RS04795
• Protein ID: WP_003404726.1
• Coordinates: 1015620..1016054 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: BCGMEX_RS04790
• Protein ID: WP_003404724.1
• Coordinates: 1015435..1015614 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGMEX_RS04780	1010908..1012488	+	1581	WP_011799139.1	serine hydrolase	-
BCGMEX_RS04785	1012485..1014878	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
BCGMEX_RS22225	1015151..1015309	+	159	WP_003404720.1	hypothetical protein	-
BCGMEX_RS04790	1015435..1015614	+	180	WP_003404724.1	antitoxin	Antitoxin
BCGMEX_RS04795	1015620..1016054	+	435	WP_003404726.1	SRPBCC family protein	Toxin
BCGMEX_RS04800	1016152..1016925	+	774	WP_003404735.1	VOC family protein	-
BCGMEX_RS04805	1016990..1017439	+	450	WP_003404738.1	hypothetical protein	-
BCGMEX_RS04810	1017574..1017804	-	231	WP_003898642.1	hypothetical protein	-
BCGMEX_RS04815	1017971..1019479	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
BCGMEX_RS04820	1019481..1020719	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T25891 WP_003404726.1 NC_016804:1015620-1016054 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T25891 NC_016804:1015620-1016054 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT25891 WP_003404724.1 NC_016804:1015435-1015614 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT25891 NC_016804:1015435-1015614 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5