Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4704282..4705092 Replicon chromosome
Accession NZ_CP104660
Organism Klebsiella pneumoniae strain 2021CK-01729

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag N4T33_RS22605 Protein ID WP_004178461.1
Coordinates 4704282..4704815 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag N4T33_RS22610 Protein ID WP_002887278.1
Coordinates 4704826..4705092 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T33_RS22600 (N4T33_22605) 4703113..4704234 + 1122 WP_002887282.1 cupin domain-containing protein -
N4T33_RS22605 (N4T33_22610) 4704282..4704815 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
N4T33_RS22610 (N4T33_22615) 4704826..4705092 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
N4T33_RS22615 (N4T33_22620) 4705195..4706628 - 1434 WP_019705874.1 Cu(+)/Ag(+) sensor histidine kinase -
N4T33_RS22620 (N4T33_22625) 4706618..4707301 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
N4T33_RS22625 (N4T33_22630) 4707473..4708858 + 1386 WP_032409188.1 efflux transporter outer membrane subunit -
N4T33_RS22630 (N4T33_22635) 4708876..4709220 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T258906 WP_004178461.1 NZ_CP104660:c4704815-4704282 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT258906 WP_002887278.1 NZ_CP104660:c4705092-4704826 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References