Detailed information of TA system TA258333

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 4249825..4250237
• Replicon: chromosome
• Accession: NZ_CP104533
• Organism: Escherichia coli strain E10

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A8E0IWC4
• Locus tag: N5O77_RS21125
• Protein ID: WP_001513534.1
• Coordinates: 4249896..4250237 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	4249825..4249901 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N5O77_RS21115 (4246437)	4246437..4247906	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
N5O77_RS21120 (4247906)	4247906..4249675	+	1770	WP_001513535.1	restriction endonuclease subunit S	-
- (4249825)	4249825..4249901	-	77	NuclAT_6	-	Antitoxin
- (4249825)	4249825..4249901	-	77	NuclAT_6	-	Antitoxin
- (4249825)	4249825..4249901	-	77	NuclAT_6	-	Antitoxin
- (4249825)	4249825..4249901	-	77	NuclAT_6	-	Antitoxin
- (4249825)	4249825..4249901	-	77	NuclAT_7	-	Antitoxin
- (4249825)	4249825..4249901	-	77	NuclAT_7	-	Antitoxin
- (4249825)	4249825..4249901	-	77	NuclAT_7	-	Antitoxin
- (4249825)	4249825..4249901	-	77	NuclAT_7	-	Antitoxin
N5O77_RS21125 (4249896)	4249896..4250237	+	342	WP_001513534.1	endoribonuclease SymE	Toxin
N5O77_RS21130 (4250284)	4250284..4251447	-	1164	WP_224153787.1	DUF1524 domain-containing protein	-
N5O77_RS21135 (4251501)	4251501..4252377	-	877	Protein_4139	DUF262 domain-containing protein	-
N5O77_RS21140 (4252783)	4252783..4253703	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
N5O77_RS21145 (4253888)	4253888..4255168	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4234213..4250237	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12247.03 Da
• Isoelectric Point: 7.8545

>T258333 WP_001513534.1 NZ_CP104533:4249896-4250237 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASHYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

Antitoxin

Nucleotide

• Length: 77 bp

>AT258333 NZ_CP104533:c4249901-4249825 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin