Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 949726..950380 Replicon chromosome
Accession NZ_CP104533
Organism Escherichia coli strain E10

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag N5O77_RS04670 Protein ID WP_000244781.1
Coordinates 949973..950380 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N5O77_RS04665 Protein ID WP_000354046.1
Coordinates 949726..949992 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O77_RS04645 (945814) 945814..947247 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
N5O77_RS04650 (947292) 947292..947603 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
N5O77_RS04655 (947767) 947767..948426 + 660 WP_000250274.1 hemolysin III family protein -
N5O77_RS04660 (948503) 948503..949483 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
N5O77_RS04665 (949726) 949726..949992 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N5O77_RS04670 (949973) 949973..950380 + 408 WP_000244781.1 protein YgfX Toxin
N5O77_RS04675 (950420) 950420..950941 - 522 WP_001055874.1 flavodoxin FldB -
N5O77_RS04680 (951053) 951053..951949 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
N5O77_RS04685 (951974) 951974..952684 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N5O77_RS04690 (952690) 952690..954423 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T258319 WP_000244781.1 NZ_CP104533:949973-950380 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT258319 WP_000354046.1 NZ_CP104533:949726-949992 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References