Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3398551..3399176 Replicon chromosome
Accession NZ_CP104523
Organism Escherichia coli strain E7

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag N5O80_RS16630 Protein ID WP_000911329.1
Coordinates 3398551..3398949 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag N5O80_RS16635 Protein ID WP_000450524.1
Coordinates 3398949..3399176 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O80_RS16610 (3394458) 3394458..3394658 + 201 WP_000383836.1 YpfN family protein -
N5O80_RS16615 (3394739) 3394739..3395437 - 699 WP_025491901.1 esterase -
N5O80_RS16620 (3395511) 3395511..3397526 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
N5O80_RS16625 (3397541) 3397541..3398404 - 864 WP_001267498.1 neutral zinc metallopeptidase -
N5O80_RS16630 (3398551) 3398551..3398949 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
N5O80_RS16635 (3398949) 3398949..3399176 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
N5O80_RS16640 (3399333) 3399333..3400046 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
N5O80_RS16645 (3400259) 3400259..3401293 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
N5O80_RS16650 (3401310) 3401310..3402188 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
N5O80_RS16655 (3402334) 3402334..3402906 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
N5O80_RS16660 (3402906) 3402906..3403376 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T258280 WP_000911329.1 NZ_CP104523:c3398949-3398551 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T258280 NZ_CP104523:c3398949-3398551 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT258280 WP_000450524.1 NZ_CP104523:c3399176-3398949 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT258280 NZ_CP104523:c3399176-3398949 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References