Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symE-timR/SymE(toxin)
Location 4163416..4163828 Replicon chromosome
Accession NZ_CP104274
Organism Escherichia coli strain Ec15103

Toxin (Protein)


Gene name symE Uniprot ID S1NWQ7
Locus tag N2O69_RS20415 Protein ID WP_000132614.1
Coordinates 4163487..4163828 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name timR
Locus tag -
Coordinates 4163416..4163492 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2O69_RS20405 (4160028) 4160028..4161497 + 1470 WP_001387312.1 type I restriction-modification system subunit M -
N2O69_RS20410 (4161497) 4161497..4163266 + 1770 WP_001513535.1 restriction endonuclease subunit S -
- (4163416) 4163416..4163492 - 77 NuclAT_8 - Antitoxin
- (4163416) 4163416..4163492 - 77 NuclAT_8 - Antitoxin
- (4163416) 4163416..4163492 - 77 NuclAT_8 - Antitoxin
- (4163416) 4163416..4163492 - 77 NuclAT_8 - Antitoxin
- (4163416) 4163416..4163492 - 77 NuclAT_9 - Antitoxin
- (4163416) 4163416..4163492 - 77 NuclAT_9 - Antitoxin
- (4163416) 4163416..4163492 - 77 NuclAT_9 - Antitoxin
- (4163416) 4163416..4163492 - 77 NuclAT_9 - Antitoxin
N2O69_RS20415 (4163487) 4163487..4163828 + 342 WP_000132614.1 endoribonuclease SymE Toxin
N2O69_RS20420 (4163875) 4163875..4165038 - 1164 WP_224153787.1 DUF1524 domain-containing protein -
N2O69_RS20425 (4165092) 4165092..4165968 - 877 Protein_3998 DUF262 domain-containing protein -
N2O69_RS20430 (4166374) 4166374..4167294 - 921 WP_001513532.1 Rpn family recombination-promoting nuclease/putative transposase -
N2O69_RS20435 (4167479) 4167479..4168759 + 1281 WP_001338077.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - cheD 4147804..4163828 16024


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12266.07 Da        Isoelectric Point: 8.4982

>T257449 WP_000132614.1 NZ_CP104274:4163487-4163828 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

Antitoxin


Download         Length: 77 bp

>AT257449 NZ_CP104274:c4163492-4163416 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1NWQ7

Antitoxin

Download structure file

References