Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 660550..661349 Replicon chromosome
Accession NZ_CP104123
Organism Escherichia coli strain USDA-ARS-USMARC-49610

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag N1705_RS03215 Protein ID WP_000347273.1
Coordinates 660550..661014 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N1705_RS03220 Protein ID WP_001307405.1
Coordinates 661014..661349 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1705_RS03185 (655551) 655551..655985 - 435 WP_219411565.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N1705_RS03190 (656003) 656003..656881 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N1705_RS03195 (656871) 656871..657650 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N1705_RS03200 (657661) 657661..658134 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N1705_RS03205 (658157) 658157..659437 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
N1705_RS03210 (659686) 659686..660495 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
N1705_RS03215 (660550) 660550..661014 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N1705_RS03220 (661014) 661014..661349 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N1705_RS03225 (661498) 661498..663069 - 1572 WP_001273771.1 galactarate dehydratase -
N1705_RS03230 (663444) 663444..664778 + 1335 WP_219411563.1 galactarate/glucarate/glycerate transporter GarP -
N1705_RS03235 (664794) 664794..665564 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T257264 WP_000347273.1 NZ_CP104123:c661014-660550 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT257264 WP_001307405.1 NZ_CP104123:c661349-661014 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References