Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 912374..912915 Replicon chromosome
Accession NZ_CP104112
Organism Escherichia coli strain USDA-ARS-USMARC-51688

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag N1707_RS04350 Protein ID WP_000615979.1
Coordinates 912374..912652 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N1707_RS04355 Protein ID WP_000729704.1
Coordinates 912655..912915 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1707_RS04335 (909956) 909956..910534 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
N1707_RS04340 (910740) 910740..911507 + 768 WP_000333379.1 class II glutamine amidotransferase -
N1707_RS04345 (911478) 911478..912218 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N1707_RS04350 (912374) 912374..912652 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N1707_RS04355 (912655) 912655..912915 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N1707_RS04360 (913101) 913101..913874 + 774 WP_001353765.1 C40 family peptidase -
N1707_RS04365 (914050) 914050..914547 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
N1707_RS04370 (914763) 914763..916463 - 1701 Protein_831 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 916478..916981 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T257194 WP_000615979.1 NZ_CP104112:c912652-912374 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT257194 WP_000729704.1 NZ_CP104112:c912915-912655 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References