Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12697..13222 Replicon plasmid pDLI.5a
Accession NZ_CP103973
Organism Escherichia coli strain DLI.5a

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag NZD79_RS24335 Protein ID WP_001159871.1
Coordinates 12917..13222 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag NZD79_RS24330 Protein ID WP_000813634.1
Coordinates 12697..12915 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZD79_RS24305 (NZD79_24305) 8218..9168 - 951 WP_032304890.1 virulence factor VirK -
NZD79_RS24310 (NZD79_24310) 9173..10261 - 1089 WP_001388979.1 putative hexosyltransferase CapU -
NZD79_RS24315 (NZD79_24315) 10264..11106 - 843 WP_160371899.1 polysaccharide deacetylase family protein -
NZD79_RS24320 (NZD79_24320) 11350..11919 - 570 WP_032304866.1 hypothetical protein -
NZD79_RS24325 (NZD79_24325) 11919..12176 - 258 WP_032304865.1 hypothetical protein -
NZD79_RS24330 (NZD79_24330) 12697..12915 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NZD79_RS24335 (NZD79_24335) 12917..13222 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
NZD79_RS24340 (NZD79_24340) 13223..14029 + 807 WP_032304863.1 site-specific integrase -
NZD79_RS24345 (NZD79_24345) 14209..14853 + 645 WP_032266348.1 ParA family protein -
NZD79_RS24350 (NZD79_24350) 14940..15248 + 309 WP_032266350.1 hypothetical protein -
NZD79_RS24355 (NZD79_24355) 15501..16198 + 698 WP_259568234.1 IS1 family transposase -
NZD79_RS24360 (NZD79_24360) 16692..17192 - 501 WP_032304856.1 helix-turn-helix domain-containing protein -
NZD79_RS24365 (NZD79_24365) 17417..18181 - 765 WP_000104222.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - etpB 1..57095 57095
- flank IS/Tn - - 15695..16198 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T256935 WP_001159871.1 NZ_CP103973:12917-13222 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT256935 WP_000813634.1 NZ_CP103973:12697-12915 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References