Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4612759..4613569 Replicon chromosome
Accession NZ_CP103729
Organism Klebsiella pneumoniae strain 197

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M5R33_RS22880 Protein ID WP_004178461.1
Coordinates 4612759..4613292 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M5R33_RS22885 Protein ID WP_002887278.1
Coordinates 4613303..4613569 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R33_RS22875 (4611590) 4611590..4612711 + 1122 WP_009309849.1 cupin domain-containing protein -
M5R33_RS22880 (4612759) 4612759..4613292 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M5R33_RS22885 (4613303) 4613303..4613569 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M5R33_RS22890 (4613672) 4613672..4615105 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
M5R33_RS22895 (4615095) 4615095..4615778 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M5R33_RS22900 (4615951) 4615951..4617336 + 1386 WP_040238382.1 efflux transporter outer membrane subunit -
M5R33_RS22905 (4617354) 4617354..4617698 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T256397 WP_004178461.1 NZ_CP103729:c4613292-4612759 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT256397 WP_002887278.1 NZ_CP103729:c4613569-4613303 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References