Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4513294..4514104 Replicon chromosome
Accession NZ_CP103727
Organism Klebsiella pneumoniae strain 2289

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M5T50_RS22245 Protein ID WP_004178461.1
Coordinates 4513294..4513827 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M5T50_RS22250 Protein ID WP_002887278.1
Coordinates 4513838..4514104 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T50_RS22240 (4512125) 4512125..4513246 + 1122 WP_012737335.1 cupin domain-containing protein -
M5T50_RS22245 (4513294) 4513294..4513827 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M5T50_RS22250 (4513838) 4513838..4514104 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M5T50_RS22255 (4514207) 4514207..4515640 - 1434 WP_029498528.1 Cu(+)/Ag(+) sensor histidine kinase -
M5T50_RS22260 (4515630) 4515630..4516313 - 684 WP_023302433.1 copper response regulator transcription factor CusR -
M5T50_RS22265 (4516485) 4516485..4517870 + 1386 WP_023302432.1 efflux transporter outer membrane subunit -
M5T50_RS22270 (4517888) 4517888..4518232 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T256382 WP_004178461.1 NZ_CP103727:c4513827-4513294 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT256382 WP_002887278.1 NZ_CP103727:c4514104-4513838 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References