Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1775671..1776502 Replicon chromosome
Accession NZ_CP103710
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag M5S69_RS08390 Protein ID WP_000854815.1
Coordinates 1775671..1776045 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag M5S69_RS08395 Protein ID WP_001280918.1
Coordinates 1776134..1776502 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S69_RS08350 (1771067) 1771067..1772233 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
M5S69_RS08355 (1772352) 1772352..1772825 + 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
M5S69_RS08360 (1773023) 1773023..1774081 + 1059 WP_001200889.1 FUSC family protein -
M5S69_RS08365 (1774253) 1774253..1774582 + 330 WP_000450409.1 DUF496 family protein -
M5S69_RS08370 (1774683) 1774683..1775006 - 324 WP_225469267.1 EutP/PduV family microcompartment system protein -
M5S69_RS08375 (1774985) 1774985..1775065 + 81 WP_023441679.1 hypothetical protein -
M5S69_RS08380 (1775354) 1775354..1775434 - 81 Protein_1638 hypothetical protein -
M5S69_RS08385 (1775480) 1775480..1775674 - 195 WP_000988600.1 DUF5983 family protein -
M5S69_RS08390 (1775671) 1775671..1776045 - 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
M5S69_RS08395 (1776134) 1776134..1776502 - 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
M5S69_RS08400 (1776518) 1776518..1777162 - 645 WP_000086752.1 hypothetical protein -
M5S69_RS08405 (1777181) 1777181..1777402 - 222 WP_000692345.1 DUF987 domain-containing protein -
M5S69_RS08410 (1777465) 1777465..1777941 - 477 WP_001186200.1 RadC family protein -
M5S69_RS08415 (1777957) 1777957..1778430 - 474 WP_001542276.1 antirestriction protein -
M5S69_RS08420 (1778524) 1778524..1778769 - 246 WP_001164966.1 hypothetical protein -
M5S69_RS08425 (1778769) 1778769..1779587 - 819 WP_001542275.1 DUF932 domain-containing protein -
M5S69_RS08430 (1779808) 1779808..1780218 - 411 WP_000846703.1 hypothetical protein -
M5S69_RS08435 (1780234) 1780234..1780584 - 351 Protein_1649 hypothetical protein -
M5S69_RS08440 (1780667) 1780667..1781413 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T256280 WP_000854815.1 NZ_CP103710:c1776045-1775671 [Escherichia coli O25b:H4-ST131]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T256280 NZ_CP103710:c1776045-1775671 [Escherichia coli O25b:H4-ST131]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT256280 WP_001280918.1 NZ_CP103710:c1776502-1776134 [Escherichia coli O25b:H4-ST131]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT256280 NZ_CP103710:c1776502-1776134 [Escherichia coli O25b:H4-ST131]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References