Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 287474..288274 Replicon chromosome
Accession NZ_CP103710
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag M5S69_RS01335 Protein ID WP_000342452.1
Coordinates 287747..288274 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag M5S69_RS01330 Protein ID WP_001277107.1
Coordinates 287474..287740 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S69_RS01310 (283133) 283133..283801 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
M5S69_RS01315 (283794) 283794..284852 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
M5S69_RS01320 (285097) 285097..285951 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
M5S69_RS01325 (286222) 286222..287325 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
M5S69_RS01330 (287474) 287474..287740 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
M5S69_RS01335 (287747) 287747..288274 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
M5S69_RS01340 (288271) 288271..288654 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
M5S69_RS01345 (289077) 289077..290186 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
M5S69_RS01350 (290234) 290234..291160 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
M5S69_RS01355 (291157) 291157..292434 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
M5S69_RS01360 (292431) 292431..293198 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T256275 WP_000342452.1 NZ_CP103710:287747-288274 [Escherichia coli O25b:H4-ST131]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT256275 WP_001277107.1 NZ_CP103710:287474-287740 [Escherichia coli O25b:H4-ST131]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References