Detailed information of TA system TA025616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 1024874..1025388
• Replicon: chromosome
• Accession: NC_015945
• Organism: Muricauda ruestringensis DSM 13258

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G2PLF4
• Locus tag: MURRU_RS04655
• Protein ID: WP_014032268.1
• Coordinates: 1025131..1025388 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: G2PLF3
• Locus tag: MURRU_RS04650
• Protein ID: WP_014032267.1
• Coordinates: 1024874..1025128 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MURRU_RS04635	1020373..1021125	+	753	WP_014032264.1	hypothetical protein	-
MURRU_RS04640	1021404..1022243	+	840	WP_014032265.1	hypothetical protein	-
MURRU_RS04645	1022580..1024724	+	2145	WP_148261560.1	carbohydrate binding family 9 domain-containing protein	-
MURRU_RS04650	1024874..1025128	+	255	WP_014032267.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MURRU_RS04655	1025131..1025388	+	258	WP_014032268.1	Txe/YoeB family addiction module toxin	Toxin
MURRU_RS04660	1025473..1026312	-	840	WP_041801313.1	rRNA pseudouridine synthase	-
MURRU_RS04665	1026373..1027278	-	906	WP_014032270.1	geranylgeranylglycerol-phosphate geranylgeranyltransferase	-
MURRU_RS04670	1027289..1028227	-	939	WP_014032271.1	mevalonate kinase	-
MURRU_RS04675	1028368..1029453	-	1086	WP_014032272.1	diphosphomevalonate decarboxylase	-
MURRU_RS04680	1029548..1030024	+	477	WP_014032273.1	tryptophan-rich sensory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10530.08 Da
• Isoelectric Point: 9.5407

>T25616 WP_014032268.1 NC_015945:1025131-1025388 [Muricauda ruestringensis DSM 13258]
MKYVFVDESWEDYLYWQKTDKKKVRKINALLKDIARNPFDGIGKPEPLKHKYSGFWSRRIDSEHRLIYQYRDEEILIAKC
RFHYD

Nucleotide

• Length: 258 bp

>T25616 NC_015945:1025131-1025388 [Muricauda ruestringensis DSM 13258]
ATGAAATATGTATTTGTGGACGAATCTTGGGAGGATTATTTGTATTGGCAAAAAACGGACAAAAAAAAGGTTAGGAAAAT
AAATGCCCTTTTAAAGGACATAGCTCGAAACCCTTTTGACGGAATTGGAAAACCAGAACCTTTAAAACACAAATACTCCG
GGTTTTGGTCAAGAAGAATTGATAGTGAACACAGACTGATATACCAATACAGAGACGAGGAAATATTGATTGCAAAATGT
AGGTTCCATTATGACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9687.09 Da
• Isoelectric Point: 7.0134

>AT25616 WP_014032267.1 NC_015945:1024874-1025128 [Muricauda ruestringensis DSM 13258]
MQITSVSDFRKDIKTYLDRVVKDFETLVINRGKDSGIVVMSLQEYNSLMATSHELSSRKNEMRLDSAIEKLKKGESFDKD
LIKD

Nucleotide

• Length: 255 bp

>AT25616 NC_015945:1024874-1025128 [Muricauda ruestringensis DSM 13258]
ATGCAGATTACGAGTGTATCGGACTTCAGAAAAGATATTAAGACCTACTTGGACAGGGTAGTGAAAGATTTTGAAACCTT
AGTCATCAACCGTGGAAAAGATTCCGGGATAGTAGTCATGTCTTTACAAGAATACAATTCATTAATGGCCACAAGCCATG
AACTATCCTCTCGAAAAAATGAAATGAGATTGGATTCCGCTATTGAGAAACTAAAAAAAGGAGAATCTTTCGATAAGGAT
CTAATCAAAGATTGA

Structures

Toxin

• AlphaFold DB: G2PLF4

Antitoxin

• AlphaFold DB: G2PLF3