Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4395707..4396542 Replicon chromosome
Accession NZ_CP103562
Organism Escherichia coli O25b:H4-ST131 strain 751

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag M5R24_RS21710 Protein ID WP_000854759.1
Coordinates 4396165..4396542 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag M5R24_RS21705 Protein ID WP_001295723.1
Coordinates 4395707..4396075 (+) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R24_RS21680 (4392822) 4392822..4393017 + 196 Protein_4256 DUF905 family protein -
M5R24_RS21685 (4393135) 4393135..4393953 + 819 WP_001234738.1 DUF932 domain-containing protein -
M5R24_RS21690 (4394295) 4394295..4394768 + 474 WP_001350782.1 antirestriction protein -
M5R24_RS21695 (4394784) 4394784..4395260 + 477 WP_001186775.1 RadC family protein -
M5R24_RS21700 (4395323) 4395323..4395544 + 222 WP_000692345.1 DUF987 domain-containing protein -
M5R24_RS21705 (4395707) 4395707..4396075 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
M5R24_RS21710 (4396165) 4396165..4396542 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
M5R24_RS21715 (4396539) 4396539..4397027 + 489 WP_000761690.1 DUF5983 family protein -
M5R24_RS21720 (4397044) 4397044..4397220 + 177 WP_000839286.1 DUF957 domain-containing protein -
M5R24_RS21725 (4397326) 4397326..4397475 + 150 Protein_4265 hypothetical protein -
M5R24_RS21730 (4397934) 4397934..4399475 + 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
M5R24_RS21735 (4399490) 4399490..4400236 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
M5R24_RS21740 (4400428) 4400428..4400604 + 177 Protein_4268 helix-turn-helix domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4382012..4410774 28762


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T255574 WP_000854759.1 NZ_CP103562:4396165-4396542 [Escherichia coli O25b:H4-ST131]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp


Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT255574 WP_001295723.1 NZ_CP103562:4395707-4396075 [Escherichia coli O25b:H4-ST131]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6


Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References