Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3727129..3727808 Replicon chromosome
Accession NZ_CP103562
Organism Escherichia coli O25b:H4-ST131 strain 751

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag M5R24_RS18515 Protein ID WP_000057523.1
Coordinates 3727506..3727808 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M5R24_RS18510 Protein ID WP_000806442.1
Coordinates 3727129..3727470 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R24_RS18500 (3723373) 3723373..3724305 - 933 WP_000883041.1 glutaminase A -
M5R24_RS18505 (3724567) 3724567..3727071 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
M5R24_RS18510 (3727129) 3727129..3727470 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M5R24_RS18515 (3727506) 3727506..3727808 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5R24_RS18520 (3727941) 3727941..3728735 + 795 WP_000365147.1 TraB/GumN family protein -
M5R24_RS18525 (3728939) 3728939..3729418 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M5R24_RS18530 (3729586) 3729586..3730242 + 657 WP_015674862.1 hypothetical protein -
M5R24_RS18535 (3730239) 3730239..3730742 + 504 WP_000667000.1 hypothetical protein -
M5R24_RS18540 (3730780) 3730780..3732432 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T255569 WP_000057523.1 NZ_CP103562:c3727808-3727506 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT255569 WP_000806442.1 NZ_CP103562:c3727470-3727129 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References