Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5433283..5433908 Replicon chromosome
Accession NZ_CP103419
Organism Klebsiella variicola strain KV4

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag NYO13_RS26160 Protein ID WP_008807903.1
Coordinates 5433283..5433666 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NYO13_RS26165 Protein ID WP_004150355.1
Coordinates 5433666..5433908 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO13_RS26145 (NYO13_26145) 5430649..5431551 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NYO13_RS26150 (NYO13_26150) 5431548..5432183 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
NYO13_RS26155 (NYO13_26155) 5432180..5433109 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NYO13_RS26160 (NYO13_26160) 5433283..5433666 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
NYO13_RS26165 (NYO13_26165) 5433666..5433908 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NYO13_RS26170 (NYO13_26170) 5434113..5435030 + 918 WP_048331367.1 alpha/beta hydrolase -
NYO13_RS26175 (NYO13_26175) 5435045..5435986 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
NYO13_RS26180 (NYO13_26180) 5436031..5436468 - 438 WP_008807906.1 D-aminoacyl-tRNA deacylase -
NYO13_RS26185 (NYO13_26185) 5436465..5437325 - 861 WP_008807907.1 virulence factor BrkB family protein -
NYO13_RS26190 (NYO13_26190) 5437319..5437918 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T255027 WP_008807903.1 NZ_CP103419:c5433666-5433283 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT255027 WP_004150355.1 NZ_CP103419:c5433908-5433666 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References