Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4617189..4617999 Replicon chromosome
Accession NZ_CP102889
Organism Klebsiella pneumoniae strain KW2

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NWT79_RS22495 Protein ID WP_004178461.1
Coordinates 4617189..4617722 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NWT79_RS22500 Protein ID WP_002887278.1
Coordinates 4617733..4617999 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT79_RS22490 (NWT79_22490) 4616020..4617141 + 1122 WP_004214138.1 cupin domain-containing protein -
NWT79_RS22495 (NWT79_22495) 4617189..4617722 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NWT79_RS22500 (NWT79_22500) 4617733..4617999 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NWT79_RS22505 (NWT79_22505) 4618102..4619535 - 1434 WP_224935433.1 Cu(+)/Ag(+) sensor histidine kinase -
NWT79_RS22510 (NWT79_22510) 4619525..4620208 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
NWT79_RS22515 (NWT79_22515) 4620381..4621766 + 1386 WP_032433446.1 efflux transporter outer membrane subunit -
NWT79_RS22520 (NWT79_22520) 4621784..4622128 + 345 WP_032433444.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T254399 WP_004178461.1 NZ_CP102889:c4617722-4617189 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT254399 WP_002887278.1 NZ_CP102889:c4617999-4617733 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References