Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4741729..4742539 Replicon chromosome
Accession NZ_CP102833
Organism Klebsiella pneumoniae strain KP4962

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NV394_RS23305 Protein ID WP_004178461.1
Coordinates 4741729..4742262 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag NV394_RS23310 Protein ID WP_004222378.1
Coordinates 4742273..4742539 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV394_RS23300 (4740560) 4740560..4741681 + 1122 WP_002887282.1 cupin domain-containing protein -
NV394_RS23305 (4741729) 4741729..4742262 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NV394_RS23310 (4742273) 4742273..4742539 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NV394_RS23315 (4742642) 4742642..4744075 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
NV394_RS23320 (4744065) 4744065..4744748 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NV394_RS23325 (4744920) 4744920..4746305 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
NV394_RS23330 (4746323) 4746323..4746667 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T254231 WP_004178461.1 NZ_CP102833:c4742262-4741729 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT254231 WP_004222378.1 NZ_CP102833:c4742539-4742273 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References