Detailed information of TA system TA025368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 3145074..3145705
• Replicon: chromosome
• Accession: NC_015656
• Organism: Frankia symbiont of Datisca glomerata

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8B3Y7
• Locus tag: FSYMDG_RS13040
• Protein ID: WP_013873915.1
• Coordinates: 3145313..3145705 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8B3Y6
• Locus tag: FSYMDG_RS13035
• Protein ID: WP_013873914.1
• Coordinates: 3145074..3145316 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSYMDG_RS13015	3140911..3142536	+	1626	WP_013873911.1	malate synthase A	-
FSYMDG_RS26205	3142694..3143251	+	558	WP_131772550.1	WD40 domain-containing protein	-
FSYMDG_RS13030	3143534..3144711	+	1178	WP_085948540.1	IS3 family transposase	-
FSYMDG_RS13035	3145074..3145316	+	243	WP_013873914.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FSYMDG_RS13040	3145313..3145705	+	393	WP_013873915.1	type II toxin-antitoxin system VapC family toxin	Toxin
FSYMDG_RS13045	3145822..3147732	-	1911	WP_157491016.1	hypothetical protein	-
FSYMDG_RS13050	3147919..3148734	-	816	WP_131772553.1	hypothetical protein	-
FSYMDG_RS13055	3148727..3149041	-	315	WP_013873916.1	hypothetical protein	-
FSYMDG_RS13060	3149196..3149969	-	774	WP_013873917.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-45)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14633.83 Da
• Isoelectric Point: 7.4013

>T25368 WP_013873915.1 NC_015656:3145313-3145705 [Candidatus Frankia datiscae]
VSRYLLDTQAWLWSLAEPRRLRADTRDLLADTANEILLSAASGWEISIKYRLGKLALPEPPRGYVPDRIRRTGVIPLAVE
HDHVLRVAELPDHHRDPFDRLLVAQAQALNVPLITADGRLSAYEVKTLPA

Nucleotide

• Length: 393 bp

>T25368 NC_015656:3145313-3145705 [Candidatus Frankia datiscae]
GTGAGCCGTTATCTGCTCGACACACAGGCATGGCTCTGGTCGCTCGCCGAGCCGCGGCGCCTGCGCGCGGACACCCGTGA
CCTGCTGGCCGACACCGCCAACGAGATCCTGCTATCGGCCGCCAGCGGCTGGGAGATTTCGATCAAATACCGGCTGGGTA
AGCTGGCCCTCCCCGAACCGCCGCGTGGCTACGTGCCGGACCGGATACGACGCACAGGTGTGATTCCGCTCGCGGTCGAG
CACGACCACGTGCTGCGGGTGGCGGAACTGCCGGACCATCACCGGGATCCCTTCGACCGGCTCCTGGTCGCGCAGGCACA
GGCACTGAACGTCCCGTTGATCACCGCGGACGGCCGGCTGTCGGCCTACGAGGTGAAAACCCTCCCAGCCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8793.96 Da
• Isoelectric Point: 4.4550

>AT25368 WP_013873914.1 NC_015656:3145074-3145316 [Candidatus Frankia datiscae]
MAKSAIQVGVHEAKTRLSELLRAVAAGQEVDILRSGEPIAKIIPFAPRSVREIGVDEGRFTVPDDFDAPLPDDIEDSFYR

Nucleotide

• Length: 243 bp

>AT25368 NC_015656:3145074-3145316 [Candidatus Frankia datiscae]
ATGGCTAAGTCCGCTATTCAGGTCGGTGTGCACGAGGCGAAGACCCGGTTGTCCGAACTGCTGCGCGCCGTAGCGGCAGG
CCAGGAGGTCGACATCCTGCGCAGCGGCGAGCCGATAGCCAAGATCATCCCGTTCGCCCCCCGGAGCGTGCGTGAGATCG
GCGTCGACGAGGGGCGGTTCACCGTCCCCGACGACTTCGACGCCCCGCTGCCCGACGACATCGAGGACAGCTTCTACCGG
TGA

Structures

Toxin

• AlphaFold DB: F8B3Y7

Antitoxin

• AlphaFold DB: F8B3Y6