Detailed information of TA system TA025363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 3224806..3225347
• Replicon: chromosome
• Accession: NC_015638
• Organism: Lacinutrix sp. 5H-3-7-4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: F6GDP7
• Locus tag: LACAL_RS14540
• Protein ID: WP_013871522.1
• Coordinates: 3225045..3225347 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F6GDP6
• Locus tag: LACAL_RS14535
• Protein ID: WP_013871521.1
• Coordinates: 3224806..3225048 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LACAL_RS14505	3220138..3220818	+	681	WP_013871517.1	hypothetical protein	-
LACAL_RS14510	3220967..3221698	+	732	WP_013871518.1	hypothetical protein	-
LACAL_RS14515	3222020..3222619	+	600	WP_148256148.1	hypothetical protein	-
LACAL_RS14520	3222809..3223174	+	366	WP_013869214.1	transposase	-
LACAL_RS14525	3223180..3224031	+	852	WP_013871510.1	IS3 family transposase	-
LACAL_RS14530	3224118..3224624	+	507	WP_013871520.1	hypothetical protein	-
LACAL_RS14535	3224806..3225048	+	243	WP_013871521.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LACAL_RS14540	3225045..3225347	+	303	WP_013871522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LACAL_RS14545	3225497..3226084	+	588	WP_013871523.1	hypothetical protein	-
LACAL_RS14550	3226243..3226716	+	474	WP_013871524.1	DUF305 domain-containing protein	-
LACAL_RS14555	3226810..3228021	-	1212	WP_013871525.1	TlpA family protein disulfide reductase	-
LACAL_RS14560	3228173..3228505	-	333	WP_013871526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3206709..3226084	19375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-94)

Antitoxin

• Antitoxin ParD (1-77)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 12307.34 Da
• Isoelectric Point: 10.2500

>T25363 WP_013871522.1 NC_015638:3225045-3225347 [Lacinutrix sp. 5H-3-7-4]
MSKNKYRISQQAIEDLNKIWIYTFHKWSKEQADRYYDLIIAEIEFIAENYLIGKSAEQTRKNYRVTKIKSHLIFYRKVEN
DIVEIVRILHQRMDIKKRLK

Nucleotide

• Length: 303 bp

>T25363 NC_015638:3225045-3225347 [Lacinutrix sp. 5H-3-7-4]
ATGAGTAAAAACAAATATCGCATAAGCCAACAAGCGATTGAAGATTTAAATAAAATTTGGATTTATACTTTTCACAAATG
GTCTAAAGAACAAGCAGACAGATATTACGATTTAATAATAGCGGAAATTGAATTTATTGCAGAGAACTATCTGATTGGAA
AATCAGCTGAACAAACTCGAAAAAATTATCGAGTAACGAAAATCAAATCACATCTGATTTTTTATAGAAAAGTTGAAAAT
GATATTGTAGAAATCGTTAGAATTTTACATCAGAGAATGGATATTAAAAAACGACTGAAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9307.43 Da
• Isoelectric Point: 6.0899

>AT25363 WP_013871521.1 NC_015638:3224806-3225048 [Lacinutrix sp. 5H-3-7-4]
MGKNTSISLGNHFEDFIREEVNSGRYGSVSEVIRSALRLLEREEKKERELIKALEVGENSGFVENFDPKQHLEELHRKHL

Nucleotide

• Length: 243 bp

>AT25363 NC_015638:3224806-3225048 [Lacinutrix sp. 5H-3-7-4]
ATGGGAAAAAACACATCAATATCACTCGGAAATCATTTTGAGGATTTTATCAGAGAAGAAGTAAATTCTGGTAGATATGG
TTCGGTTAGCGAAGTAATTCGTTCAGCATTACGCTTGTTAGAGCGCGAAGAAAAAAAGGAAAGAGAACTGATTAAAGCTC
TCGAAGTTGGAGAAAATAGTGGATTTGTTGAAAATTTTGACCCAAAACAACATCTGGAAGAATTACATCGTAAACACTTA
TGA

Structures

Toxin

• AlphaFold DB: F6GDP7

Antitoxin

• AlphaFold DB: F6GDP6