Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3578280..3578821 Replicon chromosome
Accession NZ_CP101922
Organism Escherichia coli strain JCC-EE 7

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag NP442_RS17615 Protein ID WP_000615976.1
Coordinates 3578543..3578821 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag NP442_RS17610 Protein ID WP_000729704.1
Coordinates 3578280..3578540 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP442_RS17585 (3573936) 3573936..3575648 + 1713 Protein_3438 flagellar biosynthesis protein FlhA -
NP442_RS17590 (3575872) 3575872..3576369 - 498 WP_000006242.1 REP-associated tyrosine transposase RayT -
NP442_RS17595 (3576545) 3576545..3576712 - 168 Protein_3440 C40 family peptidase -
NP442_RS17605 (3577480) 3577480..3578094 - 615 Protein_3442 C40 family peptidase -
NP442_RS17610 (3578280) 3578280..3578540 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NP442_RS17615 (3578543) 3578543..3578821 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NP442_RS17620 (3578977) 3578977..3579717 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NP442_RS17625 (3579688) 3579688..3580455 - 768 WP_001365365.1 class II glutamine amidotransferase -
NP442_RS17630 (3580661) 3580661..3581239 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T252981 WP_000615976.1 NZ_CP101922:3578543-3578821 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT252981 WP_000729704.1 NZ_CP101922:3578280-3578540 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References