Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 14399..16022 Replicon plasmid pHH194M-228K
Accession NZ_CP101516
Organism Escherichia coli strain HH194M

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag MOO21_RS23925 Protein ID WP_000088046.1
Coordinates 14399..15724 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag MOO21_RS23930 Protein ID WP_024136327.1
Coordinates 15729..16022 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO21_RS23890 (MOO21_23885) 10147..10386 - 240 Protein_12 AAC(3) family N-acetyltransferase -
MOO21_RS23895 (MOO21_23890) 10461..11486 - 1026 WP_000742814.1 aminoglycoside O-phosphotransferase APH(4)-Ia -
MOO21_RS23900 (MOO21_23895) 11715..12491 - 777 WP_001199192.1 aminoglycoside N-acetyltransferase AAC(3)-IVa -
MOO21_RS23905 (MOO21_23900) 12605..13309 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
MOO21_RS23910 (MOO21_23905) 13362..13607 - 246 Protein_16 HNH endonuclease -
MOO21_RS23915 (MOO21_23910) 13620..14030 - 411 WP_000985911.1 hypothetical protein -
MOO21_RS23920 (MOO21_23915) 14132..14338 - 207 WP_000134171.1 hypothetical protein -
MOO21_RS23925 (MOO21_23920) 14399..15724 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
MOO21_RS23930 (MOO21_23925) 15729..16022 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
MOO21_RS23935 (MOO21_23930) 16626..17018 - 393 WP_000341066.1 hypothetical protein -
MOO21_RS23940 (MOO21_23935) 17391..17732 + 342 WP_001151575.1 hypothetical protein -
MOO21_RS23945 (MOO21_23940) 17824..18015 + 192 WP_000398480.1 hypothetical protein -
MOO21_RS23950 (MOO21_23945) 18065..18346 + 282 WP_000077926.1 hypothetical protein -
MOO21_RS23955 18687..18818 - 132 WP_000374059.1 hypothetical protein -
MOO21_RS23960 (MOO21_23950) 18909..20150 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid lnu(F) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / blaNDM-4 / sul1 / qacE / aadA2 / dfrA12 / floR / blaTEM-1B - 1..228609 228609
- inside Integron lnu(F) / aadA22 / aadA2 - 1..228609 228608


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T252187 WP_000088046.1 NZ_CP101516:c15724-14399 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT252187 WP_024136327.1 NZ_CP101516:c16022-15729 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References