Detailed information of TA system TA251780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 88717..89342
• Replicon: plasmid pEC21Z014-165K
• Accession: NZ_CP101275
• Organism: Escherichia coli strain EC21Z-014

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: NML17_RS24295
• Protein ID: WP_000911333.1
• Coordinates: 88944..89342 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: NML17_RS24290
• Protein ID: WP_000450520.1
• Coordinates: 88717..88944 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NML17_RS24290 (88717)	88717..88944	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
NML17_RS24295 (88944)	88944..89342	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
NML17_RS24300 (89351)	89351..91504	-	2154	WP_000009379.1	type IV conjugative transfer system coupling protein TraD	-
NML17_RS24305 (91757)	91757..92467	-	711	WP_231793510.1	conjugal transfer complement resistance protein TraT	-
NML17_RS24310 (92502)	92502..93011	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA	1..164754	164754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T251780 WP_000911333.1 NZ_CP101275:88944-89342 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T251780 NZ_CP101275:88944-89342 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT251780 WP_000450520.1 NZ_CP101275:88717-88944 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT251780 NZ_CP101275:88717-88944 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2