Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3775938..3776592 Replicon chromosome
Accession NZ_CP101274
Organism Escherichia coli strain EC21Z-014

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag NML17_RS18405 Protein ID WP_000244772.1
Coordinates 3775938..3776345 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NML17_RS18410 Protein ID WP_000354046.1
Coordinates 3776326..3776592 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML17_RS18385 (3771895) 3771895..3773628 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
NML17_RS18390 (3773634) 3773634..3774344 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NML17_RS18395 (3774369) 3774369..3775265 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NML17_RS18400 (3775377) 3775377..3775898 + 522 WP_001055874.1 flavodoxin FldB -
NML17_RS18405 (3775938) 3775938..3776345 - 408 WP_000244772.1 protein YgfX Toxin
NML17_RS18410 (3776326) 3776326..3776592 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NML17_RS18415 (3776835) 3776835..3777815 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NML17_RS18420 (3777892) 3777892..3778551 - 660 WP_000250274.1 hemolysin III family protein -
NML17_RS18425 (3778715) 3778715..3779026 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
NML17_RS18430 (3779071) 3779071..3780504 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
NML17_RS18435 (3780561) 3780561..3781304 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T251773 WP_000244772.1 NZ_CP101274:c3776345-3775938 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT251773 WP_000354046.1 NZ_CP101274:c3776592-3776326 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References