Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1086341..1087068 Replicon chromosome
Accession NZ_CP101245
Organism Escherichia coli strain EC21Z-097

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag NML22_RS05290 Protein ID WP_000547564.1
Coordinates 1086341..1086652 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NML22_RS05295 Protein ID WP_000126294.1
Coordinates 1086649..1087068 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML22_RS05260 (1081483) 1081483..1083192 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
NML22_RS05265 (1083202) 1083202..1083744 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
NML22_RS05270 (1083744) 1083744..1084511 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NML22_RS05275 (1084508) 1084508..1084918 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
NML22_RS05280 (1084911) 1084911..1085381 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NML22_RS05285 (1085406) 1085406..1086167 + 762 WP_001026446.1 hypothetical protein -
NML22_RS05290 (1086341) 1086341..1086652 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
NML22_RS05295 (1086649) 1086649..1087068 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NML22_RS05300 (1087182) 1087182..1088606 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
NML22_RS05305 (1088615) 1088615..1090072 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NML22_RS05310 (1090332) 1090332..1091342 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
NML22_RS05315 (1091491) 1091491..1092018 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T251626 WP_000547564.1 NZ_CP101245:1086341-1086652 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT251626 WP_000126294.1 NZ_CP101245:1086649-1087068 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References