Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 316427..317227 Replicon chromosome
Accession NZ_CP101245
Organism Escherichia coli strain EC21Z-097

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NML22_RS01465 Protein ID WP_000342449.1
Coordinates 316700..317227 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NML22_RS01460 Protein ID WP_001277108.1
Coordinates 316427..316693 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML22_RS01440 (312084) 312084..312752 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NML22_RS01445 (312745) 312745..313803 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NML22_RS01450 (314048) 314048..314902 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NML22_RS01455 (315174) 315174..316277 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NML22_RS01460 (316427) 316427..316693 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NML22_RS01465 (316700) 316700..317227 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NML22_RS01470 (317224) 317224..317607 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
NML22_RS01475 (318031) 318031..319140 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NML22_RS01480 (319188) 319188..320114 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NML22_RS01485 (320111) 320111..321388 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
NML22_RS01490 (321385) 321385..322152 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T251621 WP_000342449.1 NZ_CP101245:316700-317227 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT251621 WP_001277108.1 NZ_CP101245:316427-316693 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References