250686

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4623893..4624443 Replicon chromosome
Accession NZ_CP100500
Organism Escherichia coli strain LH50-c

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag NLZ13_RS22700 Protein ID WP_001160974.1
Coordinates 4623893..4624207 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag NLZ13_RS22705 Protein ID WP_000125566.1
Coordinates 4624210..4624443 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ13_RS22675 (4619568) 4619568..4620854 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
NLZ13_RS22680 (4620854) 4620854..4621843 + 990 WP_089610127.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
NLZ13_RS22685 (4621840) 4621840..4622661 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
NLZ13_RS22690 (4622664) 4622664..4623041 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
NLZ13_RS22695 (4623048) 4623048..4623896 + 849 WP_000257188.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
NLZ13_RS22700 (4623893) 4623893..4624207 - 315 WP_001160974.1 CcdB family protein Toxin
NLZ13_RS22705 (4624210) 4624210..4624443 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
NLZ13_RS22710 (4624529) 4624529..4625008 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
NLZ13_RS22715 (4625201) 4625201..4627063 - 1863 WP_089610132.1 glutathione-regulated potassium-efflux system protein KefC -
NLZ13_RS22720 (4627056) 4627056..4627586 - 531 WP_112037758.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
NLZ13_RS22725 (4627694) 4627694..4629025 - 1332 WP_089610134.1 MFS transporter -
NLZ13_RS22730 (4629084) 4629084..4629371 - 288 WP_112037757.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T250686 WP_001160974.1 NZ_CP100500:c4624207-4623893 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT250686 WP_000125566.1 NZ_CP100500:c4624443-4624210 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References