Detailed information of TA system TA025053

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii(toxin)
• Location: 248602..250015
• Replicon: chromosome
• Accession: NC_014921
• Organism: Mycoplasma fermentans M64

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: C4XEI2
• Locus tag: MFEM64YM_RS01190
• Protein ID: WP_013354453.1
• Coordinates: 249182..250015 (+)
• Length: 278 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: -
• Locus tag: MFEM64YM_RS01185
• Protein ID: WP_013526752.1
• Coordinates: 248602..249189 (+)
• Length: 196 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MFEM64YM_RS01170	245646..246866	+	1221	WP_013526750.1	hypothetical protein	-
MFEM64YM_RS01175	247119..247763	+	645	WP_041594199.1	Fic family protein	-
MFEM64YM_RS01180	247767..248474	+	708	WP_013526751.1	B3/4 domain-containing protein	-
MFEM64YM_RS01185	248602..249189	+	588	WP_013526752.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
MFEM64YM_RS01190	249182..250015	+	834	WP_013354453.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MFEM64YM_RS01195	250064..251428	-	1365	WP_013354454.1	phosphopyruvate hydratase	-
MFEM64YM_RS01200	251663..254659	+	2997	WP_013526753.1	flippase-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-235)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 278 a.a.
• Molecular weight: 32723.18 Da
• Isoelectric Point: 9.3698

>T25053 WP_013354453.1 NC_014921:249182-250015 [Mycoplasma fermentans M64]
VNKNKLKYLCSLVSKKFDIDYNSIRKLFYLEEILKRIAKSKYRNNFIFKGGMLFSNIIGIENRRTVDIDFSLKGIESNEK
NLKIVFNEILLIKNDEINFIFNDIQPIKTDLNYPCYRFKFRASLENEKEDIVLDIGVGDKIFPKPIEYSYKSFFKDESFQ
VLSYTLETYFSEKLFAIISKGEANTRLKDYYDLHFLILNCKKMIDKSNLGKAISITFDNRNEKFDIKTAKLKLLELSNFE
LLDNKKKYLIKNEFTLNITFKNLVLMIVEFLTEIFE

Nucleotide

• Length: 834 bp

>T25053 NC_014921:249182-250015 [Mycoplasma fermentans M64]
GTGAATAAAAATAAATTGAAATATTTGTGTTCCTTGGTTTCAAAGAAATTTGATATTGATTACAATTCCATAAGAAAATT
ATTTTATCTTGAAGAAATACTAAAAAGAATTGCAAAAAGTAAATATAGAAATAATTTTATTTTTAAAGGCGGGATGTTGT
TTTCAAACATAATAGGTATTGAAAATAGAAGAACTGTTGATATTGATTTTAGTTTGAAAGGTATAGAGTCTAATGAAAAA
AATTTAAAAATTGTTTTTAATGAAATATTGTTAATAAAAAATGATGAAATAAATTTTATTTTTAATGATATACAACCTAT
AAAAACTGATTTAAATTATCCTTGTTACAGATTTAAATTTAGAGCTTCCTTAGAAAACGAGAAAGAAGATATTGTTCTTG
ATATTGGTGTTGGAGATAAAATATTTCCAAAACCAATTGAATACTCTTATAAAAGTTTTTTTAAAGATGAAAGTTTTCAA
GTTTTGTCATACACTCTTGAAACATATTTTTCAGAAAAACTTTTTGCAATAATTTCTAAAGGAGAAGCTAATACAAGACT
AAAAGATTATTATGATTTACATTTTTTAATATTAAATTGTAAAAAAATGATTGATAAATCAAATTTAGGCAAAGCAATTT
CGATAACATTTGATAATAGAAATGAAAAATTTGACATTAAAACTGCAAAATTAAAACTTTTAGAGTTATCTAATTTTGAG
TTATTGGATAATAAATGAAAAAAATATTTAATCAAAAATGAATTTACTTTAAATATTACTTTTAAAAATTTAGTTTTAAT
GATTGTTGAATTTTTAACTGAAATTTTTGAATAA

Antitoxin

Protein

• Length: 196 a.a.
• Molecular weight: 22582.07 Da
• Isoelectric Point: 8.9364

>AT25053 WP_013526752.1 NC_014921:248602-249189 [Mycoplasma fermentans M64]
MNYNEKLEKVIEECNGVVTTKECNKRNIPNSIISRAAKKGRIEKYSEGIYVAEEIPMDPHFLFQLRFPRSIFSYESALQL
HGVSERFEKALEVTVNTGYKFNDTKKIFPHYVKDEYLELGVEEIKTSQGNKVRVYSLERTFCDMVANRNKVDAETFVKFM
ITFQGIKINYSQLNSIAKKMGVINKVVDVMGIIRE

Nucleotide

• Length: 588 bp

>AT25053 NC_014921:248602-249189 [Mycoplasma fermentans M64]
ATGAACTACAATGAAAAACTTGAAAAAGTTATTGAAGAATGTAATGGAGTTGTTACTACTAAAGAATGTAATAAAAGAAA
TATTCCTAATTCAATAATTTCAAGAGCTGCCAAAAAAGGCAGAATTGAAAAATATTCTGAAGGAATTTATGTTGCTGAAG
AAATACCAATGGATCCACACTTTCTTTTTCAACTTCGATTTCCAAGAAGTATTTTTTCTTATGAAAGTGCATTGCAACTT
CATGGAGTGAGTGAAAGATTTGAAAAAGCGCTAGAAGTCACAGTAAATACAGGATATAAATTTAATGATACTAAGAAAAT
CTTTCCGCACTATGTAAAAGATGAGTATCTTGAATTGGGAGTAGAAGAAATAAAAACAAGTCAAGGCAATAAGGTTAGAG
TTTATTCTTTGGAAAGAACTTTTTGCGATATGGTAGCTAATAGAAATAAAGTAGATGCTGAAACTTTTGTAAAATTTATG
ATTACATTCCAAGGAATAAAAATAAATTATTCTCAACTTAATAGTATTGCTAAAAAAATGGGAGTCATTAACAAAGTTGT
TGATGTAATGGGAATAATTCGTGAATAA

Structures

Toxin

• AlphaFold DB: C4XEI2