Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3441833..3442470 Replicon chromosome
Accession NZ_CP100391
Organism Bacillus velezensis strain PHP1601

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NJ242_RS16955 Protein ID WP_003156187.1
Coordinates 3441833..3442183 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NJ242_RS16960 Protein ID WP_003156188.1
Coordinates 3442189..3442470 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NJ242_RS16915 (NJ242_16915) 3436873..3437475 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
NJ242_RS16920 (NJ242_16920) 3437475..3438263 - 789 WP_032873003.1 RNA polymerase sigma factor SigB -
NJ242_RS16925 (NJ242_16925) 3438229..3438711 - 483 WP_007609591.1 anti-sigma B factor RsbW -
NJ242_RS16930 (NJ242_16930) 3438708..3439037 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NJ242_RS16935 (NJ242_16935) 3439101..3440108 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
NJ242_RS16940 (NJ242_16940) 3440120..3440521 - 402 WP_003156178.1 anti-sigma regulatory factor -
NJ242_RS16945 (NJ242_16945) 3440524..3440889 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
NJ242_RS16950 (NJ242_16950) 3440894..3441715 - 822 WP_003156182.1 STAS domain-containing protein -
NJ242_RS16955 (NJ242_16955) 3441833..3442183 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NJ242_RS16960 (NJ242_16960) 3442189..3442470 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NJ242_RS16965 (NJ242_16965) 3442590..3443759 - 1170 WP_032873001.1 alanine racemase -
NJ242_RS16970 (NJ242_16970) 3443876..3444883 - 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
NJ242_RS16975 (NJ242_16975) 3445048..3445413 - 366 WP_007609580.1 holo-ACP synthase -
NJ242_RS16980 (NJ242_16980) 3445506..3446105 + 600 WP_032872997.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T250521 WP_003156187.1 NZ_CP100391:c3442183-3441833 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT250521 WP_003156188.1 NZ_CP100391:c3442470-3442189 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References