Detailed information of TA system TA025002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 613423..613987
• Replicon: chromosome
• Accession: NC_014816
• Organism: Asticcacaulis excentricus CB 48

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E8RR02
• Locus tag: ASTEX_RS02850
• Protein ID: WP_013478099.1
• Coordinates: 613423..613704 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E8RR03
• Locus tag: ASTEX_RS02855
• Protein ID: WP_013478100.1
• Coordinates: 613691..613987 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASTEX_RS02830	609916..610617	+	702	WP_013478095.1	response regulator transcription factor	-
ASTEX_RS02835	610614..611993	+	1380	WP_013478096.1	HAMP domain-containing histidine kinase	-
ASTEX_RS02840	611990..612532	+	543	WP_049781588.1	hypothetical protein	-
ASTEX_RS02845	612529..612972	-	444	WP_013478098.1	hypothetical protein	-
ASTEX_RS02850	613423..613704	-	282	WP_013478099.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASTEX_RS02855	613691..613987	-	297	WP_013478100.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ASTEX_RS02860	614316..615989	-	1674	WP_013478101.1	glutamate--tRNA ligase	-
ASTEX_RS02870	616272..617300	-	1029	WP_013478102.1	glycosyltransferase	-
ASTEX_RS02875	617631..618122	+	492	WP_013478103.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-52)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10819.56 Da
• Isoelectric Point: 9.9872

>T25002 WP_013478099.1 NC_014816:c613704-613423 [Asticcacaulis excentricus CB 48]
VLALEWRSAARADLLAIIDYISDNNPDAAQRLKDIIETKVAKLQHRLKLFRLGRVSETREMTVRPIYVVIYTFDAEKIST
LRVLHTARQWPET

Nucleotide

• Length: 282 bp

>T25002 NC_014816:c613704-613423 [Asticcacaulis excentricus CB 48]
GTGCTGGCGCTTGAATGGCGTTCGGCGGCGCGCGCAGACCTTCTCGCGATAATCGATTACATCTCTGACAATAATCCAGA
TGCCGCGCAGCGCTTGAAAGATATCATCGAGACCAAGGTAGCGAAACTCCAACATCGGCTAAAGTTGTTTCGACTGGGCA
GAGTATCGGAAACACGCGAAATGACGGTTCGTCCCATCTACGTAGTAATATACACTTTCGACGCTGAGAAGATATCGACT
CTGAGAGTGCTCCATACCGCACGACAATGGCCTGAAACCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10789.23 Da
• Isoelectric Point: 4.8668

>AT25002 WP_013478100.1 NC_014816:c613987-613691 [Asticcacaulis excentricus CB 48]
MAQTSMLHVRIDDELKAAAAEKLADFGLTISDAARILLTREAKYGGLPAGLMTSPEAYDEWFRAKVYEALEDEGPGINHQ
QVMNEAQALIDRKRRAGA

Nucleotide

• Length: 297 bp

>AT25002 NC_014816:c613987-613691 [Asticcacaulis excentricus CB 48]
ATGGCCCAGACTTCCATGCTTCACGTTCGAATCGACGACGAACTTAAAGCGGCTGCCGCTGAGAAACTCGCTGACTTCGG
GCTAACCATCTCCGATGCCGCACGCATCTTGCTCACTCGTGAAGCAAAGTACGGCGGGCTGCCAGCTGGTCTCATGACTT
CACCAGAAGCCTATGATGAGTGGTTTAGAGCGAAGGTCTATGAAGCTCTTGAGGACGAAGGTCCCGGCATCAATCATCAG
CAGGTGATGAACGAAGCCCAAGCTCTTATTGATAGGAAGCGTCGTGCTGGCGCTTGA

Structures

Toxin

• AlphaFold DB: E8RR02

Antitoxin

• AlphaFold DB: E8RR03