Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3041645..3042305 Replicon chromosome
Accession NZ_CP099375
Organism Citrobacter braakii strain RHB25-E4-C05

Toxin (Protein)


Gene name higB Uniprot ID A0A2I8S535
Locus tag NFK28_RS14890 Protein ID WP_053390179.1
Coordinates 3041952..3042305 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NFK28_RS14885 Protein ID WP_053390178.1
Coordinates 3041645..3041947 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK28_RS14865 (3037621) 3037621..3037773 + 153 WP_165500029.1 hypothetical protein -
NFK28_RS14875 (3038124) 3038124..3039386 + 1263 WP_053390176.1 integrase arm-type DNA-binding domain-containing protein -
NFK28_RS14880 (3040259) 3040259..3041146 - 888 WP_053390177.1 integrase domain-containing protein -
NFK28_RS14885 (3041645) 3041645..3041947 - 303 WP_053390178.1 XRE family transcriptional regulator Antitoxin
NFK28_RS14890 (3041952) 3041952..3042305 - 354 WP_053390179.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFK28_RS14895 (3042804) 3042804..3043025 - 222 WP_053390180.1 helix-turn-helix transcriptional regulator -
NFK28_RS14900 (3043022) 3043022..3043489 - 468 WP_053390181.1 hypothetical protein -
NFK28_RS14905 (3044003) 3044003..3045211 + 1209 WP_149330251.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3036643..3060258 23615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-81)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13442.36 Da        Isoelectric Point: 9.0134

>T248710 WP_053390179.1 NZ_CP099375:c3042305-3041952 [Citrobacter braakii]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Download         Length: 354 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11040.86 Da        Isoelectric Point: 6.4738

>AT248710 WP_053390178.1 NZ_CP099375:c3041947-3041645 [Citrobacter braakii]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S535

Antitoxin

Source ID Structure

References