Detailed information of TA system TA024794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 403229..403815
• Replicon: chromosome
• Accession: NC_014228
• Organism: Xenorhabdus nematophila ATCC 19061

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D3VIJ6
• Locus tag: XNC1_RS02035
• Protein ID: WP_013183288.1
• Coordinates: 403486..403815 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D3VIJ5
• Locus tag: XNC1_RS02030
• Protein ID: WP_013183287.1
• Coordinates: 403229..403486 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XNC1_RS01995 (XNC1_0464)	398388..399125	+	738	WP_013183284.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
XNC1_RS02000 (XNC1_0465)	399323..399721	+	399	WP_010845247.1	30S ribosomal protein S6	-
XNC1_RS02005 (XNC1_0466)	399727..400044	+	318	WP_010845246.1	primosomal replication protein N	-
XNC1_RS02010 (XNC1_0467)	400049..400276	+	228	WP_010845245.1	30S ribosomal protein S18	-
XNC1_RS02015 (XNC1_0468)	400315..400767	+	453	WP_013183285.1	50S ribosomal protein L9	-
XNC1_RS02020 (XNC1_0469)	400902..401819	-	918	WP_013183286.1	glycerophosphoryl diester phosphodiesterase	-
XNC1_RS02025 (XNC1_0470)	402374..402994	+	621	WP_010845240.1	peptidylprolyl isomerase	-
XNC1_RS02030 (XNC1_0471)	403229..403486	+	258	WP_013183287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
XNC1_RS02035 (XNC1_0472)	403486..403815	+	330	WP_013183288.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XNC1_RS02040 (XNC1_0473)	403985..404728	+	744	WP_010845236.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
XNC1_RS02045 (XNC1_0474)	404820..405377	-	558	WP_010845235.1	YtfJ family protein	-
XNC1_RS02050 (XNC1_0475)	405704..405910	+	207	WP_010845234.1	DUF1107 domain-containing protein	-
XNC1_RS02055 (XNC1_0476)	406025..407356	-	1332	WP_010845233.1	hemolysin family protein	-
XNC1_RS02060 (XNC1_0477)	407442..408089	-	648	WP_013183289.1	peptide-methionine (S)-S-oxide reductase MsrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• SpoVT-AbrB domain (13-47)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12349.57 Da
• Isoelectric Point: 9.9619

>T24794 WP_013183288.1 NC_014228:403486-403815 [Xenorhabdus nematophila ATCC 19061]
MYIPDKGDIVSLNFDPSAGKEIMKRRPVFVISRKMFNERTGFAVVVPITSTARGMKLEVVLPEEVSVQGSILIHQVKSLD
FSERQVKFIEKAPQYIIDRVTELTKAIIL

Nucleotide

• Length: 330 bp

>T24794 NC_014228:403486-403815 [Xenorhabdus nematophila ATCC 19061]
ATGTATATACCAGACAAAGGTGACATTGTTTCATTAAACTTTGATCCGAGTGCAGGCAAGGAAATCATGAAACGCCGTCC
TGTATTCGTTATATCTCGTAAAATGTTTAACGAGCGCACTGGATTTGCTGTTGTAGTTCCAATCACGAGTACAGCAAGAG
GAATGAAGCTAGAAGTTGTCTTACCCGAAGAAGTATCTGTTCAAGGCTCAATTCTCATCCATCAGGTAAAATCCTTAGAT
TTTTCGGAGCGTCAGGTCAAATTTATTGAGAAAGCGCCTCAATATATTATTGATAGAGTGACTGAGCTAACTAAGGCCAT
CATCTTATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9273.67 Da
• Isoelectric Point: 4.5959

>AT24794 WP_013183287.1 NC_014228:403229-403486 [Xenorhabdus nematophila ATCC 19061]
MTAVVIRQSGGANIVSIPKAIVKTLNLHTGSKLELSIQDNKIVLTPVEEELTLESLLAGSPKECFRVTDEDREWLEANSV
GKEVI

Nucleotide

• Length: 258 bp

>AT24794 NC_014228:403229-403486 [Xenorhabdus nematophila ATCC 19061]
ATGACTGCTGTTGTCATTCGCCAATCTGGCGGTGCTAACATTGTTAGCATTCCAAAGGCCATAGTGAAAACACTTAATCT
GCATACTGGCTCAAAGCTAGAGCTATCTATTCAGGATAACAAAATTGTACTGACTCCAGTCGAAGAAGAGTTGACGCTGG
AATCGCTGTTAGCAGGAAGCCCTAAAGAATGTTTCAGAGTCACAGATGAGGATAGAGAGTGGCTTGAGGCTAATTCTGTT
GGTAAGGAAGTGATTTGA

Structures

Toxin

• AlphaFold DB: D3VIJ6

Antitoxin

• AlphaFold DB: D3VIJ5