Detailed information of TA system TA024781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2565951..2566538
• Replicon: chromosome
• Accession: NC_014216
• Organism: Desulfurivibrio alkaliphilus AHT2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D6Z6A0
• Locus tag: DAAHT2_RS11165
• Protein ID: WP_013164379.1
• Coordinates: 2565951..2566232 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: DAAHT2_RS11170
• Protein ID: WP_013164380.1
• Coordinates: 2566248..2566538 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DAAHT2_RS11155	2561876..2563870	-	1995	WP_013164377.1	acetate--CoA ligase	-
DAAHT2_RS11160	2564131..2565435	-	1305	WP_013164378.1	porin	-
DAAHT2_RS14725	2565598..2565852	+	255	WP_157861473.1	hypothetical protein	-
DAAHT2_RS11165	2565951..2566232	+	282	WP_013164379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DAAHT2_RS11170	2566248..2566538	+	291	WP_013164380.1	HigA family addiction module antidote protein	Antitoxin
DAAHT2_RS11175	2566578..2567294	+	717	WP_013164381.1	orotidine-5'-phosphate decarboxylase	-
DAAHT2_RS11180	2567315..2568742	-	1428	WP_013164382.1	FAD-binding protein	-
DAAHT2_RS11185	2568776..2569225	-	450	WP_013164383.1	hypothetical protein	-
DAAHT2_RS11190	2569212..2569679	-	468	WP_013164384.1	PTS sugar transporter subunit IIA	-
DAAHT2_RS11195	2569783..2571279	-	1497	WP_013164385.1	RNA polymerase factor sigma-54	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-94)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10830.43 Da
• Isoelectric Point: 10.4524

>T24781 WP_013164379.1 NC_014216:2565951-2566232 [Desulfurivibrio alkaliphilus AHT 2]
MIVSIKDSETAKVWAGYFSRKLPTSIQAVARRKLRMINNARAIDDLRIPPANRLEKLSGNRAGQWSIRINDQWRICFTWN
NGQVEQVEICDYH

Nucleotide

• Length: 282 bp

>T24781 NC_014216:2565951-2566232 [Desulfurivibrio alkaliphilus AHT 2]
ATGATTGTCTCTATCAAGGACAGTGAAACGGCCAAAGTTTGGGCGGGCTATTTTTCTCGCAAACTGCCAACTTCCATTCA
GGCGGTTGCTCGGCGGAAGTTGAGAATGATTAACAACGCTCGGGCAATTGATGATCTGCGAATTCCGCCCGCAAATCGCC
TGGAAAAGCTTTCCGGTAACCGGGCCGGCCAGTGGAGCATCAGAATCAACGACCAATGGCGAATATGCTTTACCTGGAAC
AACGGCCAGGTCGAGCAGGTTGAGATTTGCGATTACCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10336.88 Da
• Isoelectric Point: 6.9783

>AT24781 WP_013164380.1 NC_014216:2566248-2566538 [Desulfurivibrio alkaliphilus AHT 2]
MERLPNIHPGEVLREEFLKPLGISQYRLAKAIGVSPMRISEICAGKRSVTADSAIRLARALGTTPGFWLGLQADYDTEEA
INAAGNTLDAISRLAA

Nucleotide

• Length: 291 bp

>AT24781 NC_014216:2566248-2566538 [Desulfurivibrio alkaliphilus AHT 2]
ATGGAAAGACTTCCCAACATCCACCCCGGTGAGGTTTTGCGCGAAGAGTTCCTTAAGCCTTTGGGCATCAGTCAGTACCG
ACTGGCCAAAGCCATCGGGGTATCACCCATGCGAATCAGCGAGATTTGCGCCGGCAAGCGCTCGGTTACCGCCGACAGCG
CCATCAGGCTGGCCCGGGCGCTGGGAACCACTCCCGGTTTCTGGCTGGGCCTGCAAGCCGACTACGACACCGAAGAGGCC
ATCAACGCCGCCGGCAACACCCTGGACGCCATCAGTCGGCTGGCCGCATAA

Structures

Toxin

• AlphaFold DB: D6Z6A0