Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 647841..648357 Replicon chromosome
Accession NC_014121
Organism Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)


Gene name relE Uniprot ID A0A0M7F3X9
Locus tag ECL_RS03145 Protein ID WP_013095355.1
Coordinates 648073..648357 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V3EXX3
Locus tag ECL_RS03140 Protein ID WP_008501400.1
Coordinates 647841..648083 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECL_RS03125 643831..644571 + 741 WP_013095353.1 KDGP aldolase family protein -
ECL_RS03130 644695..645834 + 1140 WP_100168201.1 lactonase family protein -
ECL_RS03135 645854..647763 + 1910 Protein_609 BglG family transcription antiterminator -
ECL_RS03140 647841..648083 + 243 WP_008501400.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
ECL_RS03145 648073..648357 + 285 WP_013095355.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECL_RS03150 648361..648825 - 465 WP_013095356.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
ECL_RS03155 648948..651086 - 2139 WP_013095357.1 anaerobic ribonucleoside-triphosphate reductase -
ECL_RS03160 651460..653103 - 1644 WP_013095358.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10986.92 Da        Isoelectric Point: 10.4251

>T24739 WP_013095355.1 NC_014121:648073-648357 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MTYELEFDPRALKEWSKLGETVKKQFKKKLAGVLVNPRIESARLHNLPDCYKIKLRSQGYRLVYQVQDNVVTVVVIAIGK
REKLTVYHDANKRL

Download         Length: 285 bp

>T24739 NC_014121:648073-648357 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAAGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAA
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCTGCATAACCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCATCGCCATTGGGAAA
AGAGAGAAATTAACTGTGTATCACGATGCTAACAAACGGCTATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8972.37 Da        Isoelectric Point: 5.0651

>AT24739 WP_008501400.1 NC_014121:647841-648083 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Download         Length: 243 bp

>AT24739 NC_014121:647841-648083 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTGTTTCAGTATGTCGCCGAGACGGGACGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M7F3X9

Antitoxin

Source ID Structure
AlphaFold DB V3EXX3

References