Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 176041..176645 Replicon chromosome
Accession NC_014121
Organism Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)


Gene name hicA Uniprot ID A0A421IJD5
Locus tag ECL_RS00865 Protein ID WP_071843252.1
Coordinates 176041..176226 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7Z1F6W0
Locus tag ECL_RS00870 Protein ID WP_013094943.1
Coordinates 176241..176645 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECL_RS00850 172472..174010 + 1539 WP_013094940.1 aldehyde dehydrogenase -
ECL_RS00855 174007..174972 - 966 WP_013094941.1 LysR family transcriptional regulator -
ECL_RS00860 175090..175830 + 741 WP_013094942.1 MipA/OmpV family protein -
ECL_RS00865 176041..176226 + 186 WP_071843252.1 type II toxin-antitoxin system HicA family toxin Toxin
ECL_RS00870 176241..176645 + 405 WP_013094943.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECL_RS00875 176699..178654 - 1956 WP_013094944.1 glycoside hydrolase family 127 protein -
ECL_RS00880 178665..180017 - 1353 WP_013094945.1 MFS transporter -
ECL_RS00885 180242..181060 + 819 WP_013094946.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6859.08 Da        Isoelectric Point: 11.7891

>T24736 WP_071843252.1 NC_014121:176041-176226 [Enterobacter cloacae subsp. cloacae ATCC 13047]
VKSADVITVLVRHGWKCVRTKGSHHQFRHPVHAGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T24736 NC_014121:176041-176226 [Enterobacter cloacae subsp. cloacae ATCC 13047]
GTGAAAAGTGCGGATGTCATTACTGTTCTGGTTAGGCATGGCTGGAAATGTGTAAGAACGAAAGGAAGCCATCATCAGTT
TCGCCACCCAGTACATGCGGGGTTGGTGACCGTTCCACATCCCAAAAAGGACATAAAACCCGGCACCCTCGCGCAAATCT
GGCGGCAGGCGGGAATCAAACACTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14915.74 Da        Isoelectric Point: 4.3036

>AT24736 WP_013094943.1 NC_014121:176241-176645 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MFYPAYIHSDPDGSASGFFPDVPGCFFAGNSLDDAFQDARDALTAHFEALFEMDEELPLPGNVEAHLEATPGDFIGGQWL
LVDINMKQFDGKAERINITLPRRLLVKIDSFVNEHPQFSNRSAFLAEAARRVLP

Download         Length: 405 bp

>AT24736 NC_014121:176241-176645 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGTTTTATCCGGCTTACATTCATTCTGATCCCGACGGTAGCGCCAGCGGTTTTTTTCCTGACGTTCCTGGTTGTTTTTT
TGCCGGCAATTCTCTGGATGATGCTTTTCAGGATGCACGTGATGCACTGACAGCTCACTTCGAAGCGCTATTTGAAATGG
ATGAAGAGTTACCTCTCCCTGGCAATGTTGAAGCACATCTTGAAGCCACACCGGGTGATTTTATCGGCGGACAATGGTTG
CTTGTAGATATCAATATGAAGCAATTCGACGGTAAGGCCGAGCGTATCAATATCACCCTGCCTCGTCGATTGCTGGTGAA
AATTGATTCTTTTGTGAATGAGCATCCGCAGTTTAGTAACCGAAGCGCTTTTCTTGCTGAGGCAGCGCGTCGCGTGCTGC
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421IJD5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1F6W0

References