Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3825411..3826138 Replicon chromosome
Accession NZ_CP098217
Organism Escherichia coli strain Z0117EC0032

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NBY14_RS18125 Protein ID WP_000547555.1
Coordinates 3825411..3825722 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NBY14_RS18130 Protein ID WP_000126294.1
Coordinates 3825719..3826138 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY14_RS18100 (3821346) 3821346..3823055 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
NBY14_RS18105 (3823065) 3823065..3823607 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NBY14_RS18110 (3823607) 3823607..3824374 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NBY14_RS18115 (3824371) 3824371..3824781 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NBY14_RS18120 (3824774) 3824774..3825244 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
NBY14_RS18125 (3825411) 3825411..3825722 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NBY14_RS18130 (3825719) 3825719..3826138 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NBY14_RS18135 (3826217) 3826217..3827641 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NBY14_RS18140 (3827650) 3827650..3829107 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NBY14_RS18145 (3829367) 3829367..3830377 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NBY14_RS18150 (3830526) 3830526..3831053 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T247172 WP_000547555.1 NZ_CP098217:3825411-3825722 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT247172 WP_000126294.1 NZ_CP098217:3825719-3826138 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References