Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1266238..1266965 Replicon chromosome
Accession NZ_CP098197
Organism Escherichia coli strain Z0117EC0062

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NBY21_RS06185 Protein ID WP_000547555.1
Coordinates 1266238..1266549 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NBY21_RS06190 Protein ID WP_000126294.1
Coordinates 1266546..1266965 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY21_RS06160 (1262173) 1262173..1263882 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
NBY21_RS06165 (1263892) 1263892..1264434 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NBY21_RS06170 (1264434) 1264434..1265201 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NBY21_RS06175 (1265198) 1265198..1265608 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NBY21_RS06180 (1265601) 1265601..1266071 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
NBY21_RS06185 (1266238) 1266238..1266549 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NBY21_RS06190 (1266546) 1266546..1266965 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NBY21_RS06195 (1267044) 1267044..1268468 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NBY21_RS06200 (1268477) 1268477..1269934 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NBY21_RS06205 (1270194) 1270194..1271204 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NBY21_RS06210 (1271353) 1271353..1271880 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T246969 WP_000547555.1 NZ_CP098197:1266238-1266549 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT246969 WP_000126294.1 NZ_CP098197:1266546-1266965 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References