Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1025049..1025703 Replicon chromosome
Accession NZ_CP098195
Organism Escherichia coli strain Z0117EC0098

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag NBY23_RS05060 Protein ID WP_000244772.1
Coordinates 1025296..1025703 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NBY23_RS05055 Protein ID WP_000354046.1
Coordinates 1025049..1025315 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY23_RS05030 (1020337) 1020337..1021080 + 744 WP_000951964.1 SDR family oxidoreductase -
NBY23_RS05035 (1021137) 1021137..1022570 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
NBY23_RS05040 (1022615) 1022615..1022926 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
NBY23_RS05045 (1023090) 1023090..1023749 + 660 WP_000250274.1 hemolysin III family protein -
NBY23_RS05050 (1023826) 1023826..1024806 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NBY23_RS05055 (1025049) 1025049..1025315 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NBY23_RS05060 (1025296) 1025296..1025703 + 408 WP_000244772.1 protein YgfX Toxin
NBY23_RS05065 (1025743) 1025743..1026264 - 522 WP_001055874.1 flavodoxin FldB -
NBY23_RS05070 (1026376) 1026376..1027272 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NBY23_RS05075 (1027297) 1027297..1028007 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NBY23_RS05080 (1028013) 1028013..1029746 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T246940 WP_000244772.1 NZ_CP098195:1025296-1025703 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT246940 WP_000354046.1 NZ_CP098195:1025049-1025315 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References