Detailed information of TA system TA246933

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 10184..10610
• Replicon: plasmid pZ0117ECO0116-2
• Accession: NZ_CP098194
• Organism: Escherichia coli strain Z0117EC0116

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: NBY24_RS24880
• Protein ID: WP_001372321.1
• Coordinates: 10184..10309 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	10386..10610 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NBY24_RS24845 (5207)	5207..5434	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
NBY24_RS24850 (5571)	5571..6242	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
NBY24_RS24855 (6436)	6436..6819	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
NBY24_RS24860 (7142)	7142..7744	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
NBY24_RS24865 (8041)	8041..8862	-	822	WP_001234445.1	DUF932 domain-containing protein	-
NBY24_RS24870 (8973)	8973..9269	-	297	WP_001272251.1	hypothetical protein	-
NBY24_RS24875 (9569)	9569..9865	+	297	Protein_16	hypothetical protein	-
NBY24_RS24880 (10184)	10184..10309	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
NBY24_RS24885 (10251)	10251..10400	-	150	Protein_18	plasmid maintenance protein Mok	-
NBY24_RS24890 (10422)	10422..10601	+	180	WP_001309233.1	hypothetical protein	-
- (10386)	10386..10610	-	225	NuclAT_0	-	Antitoxin
- (10386)	10386..10610	-	225	NuclAT_0	-	Antitoxin
- (10386)	10386..10610	-	225	NuclAT_0	-	Antitoxin
- (10386)	10386..10610	-	225	NuclAT_0	-	Antitoxin
NBY24_RS24895 (10579)	10579..11341	-	763	Protein_20	plasmid SOS inhibition protein A	-
NBY24_RS24900 (11338)	11338..11772	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
NBY24_RS24905 (11841)	11841..13864	-	2024	Protein_22	ParB/RepB/Spo0J family partition protein	-
NBY24_RS24910 (13925)	13925..14158	-	234	WP_000006003.1	DUF905 family protein	-
NBY24_RS24915 (14216)	14216..14743	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
NBY24_RS24920 (15045)	15045..15494	+	450	Protein_25	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T246933 WP_001372321.1 NZ_CP098194:c10309-10184 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT246933 NZ_CP098194:c10610-10386 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin